Seasonal variations in the seminal plasma composition of male goats

Authors

  • Julio Cesar Oliveira Dias Universidade Federal de Viçosa http://orcid.org/0000-0002-9356-4264
  • Cristina Mattos Veloso
  • Madriano Christilis da Rocha Santos Santos
  • Carlos Thiago Santos Alvim Mendes de Oliveira
  • Camila Oliveira Silveira
  • Erick Iglesias
  • Pedro Henrique Furtado
  • Rita Flávia Miranda de Oliveira Donzele

DOI:

https://doi.org/10.33837/msj.v1i7.339

Keywords:

eletroforese, minerais, proteínas, espermatozoide

Abstract

Este estudo avaliou as variações mensais proteicas e bioquímicas no plasma seminal de bodes da raça Alpina criados em clima tropical. A avaliação da motilidade progressiva de espermatozóides foi realizada no sêmen fresco e descongelado, e a avaliação da funcionalidade da membrana após a descongelação. Após 12 meses, o plasma seminal foi submetido a eletroforese em gel de SDS-poliacrilamida a 10 e 14%, em paralelo com as análises dos seus parâmetros bioquímicos. No gel de poliacrilamida a 10% foram identificadas 22 bandas proteicas  (25-181 kDa), enquanto que no gel de 14%, foram identificadas 16 bandas proteicas (5,7-165 kDa). As frações de proteínas de 5,7 e 34,3-34,5 kDa mostraram um perfil que variou de acordo com a sazonalidade reprodutiva dos bodes, com o aumento da produção ocorrendo durante a estação reprodutiva. Na análise dos parâmetros bioquímicos, as concentrações de cálcio, fósforo, e colesterol também mostraram variação sazonal. No entanto, a análise da motilidade progressiva, bem como a funcionalidade de membrana não alteraram. Estes resultados indicam que alterações nos perfis proteico e bioquímico do plasma seminal durante o ano, não alterou a qualidade do sêmen fresco e descongelado de bodes Alpinos em clima tropical.

Author Biography

Julio Cesar Oliveira Dias, Universidade Federal de Viçosa

Médico Veterinário atuando na área de Reprodução Animal (Fisiologia e Biotecnologias), com ênfase em ruminantes (bovinos, caprinos e ovinos)

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Published

2018-03-18

How to Cite

Dias, J. C. O., Veloso, C. M., Santos, M. C. da R. S., de Oliveira, C. T. S. A. M., Silveira, C. O., Iglesias, E., Furtado, P. H., & Donzele, R. F. M. de O. (2018). Seasonal variations in the seminal plasma composition of male goats. Multi-Science Journal (ISSN 2359-6902), 1(7), 34-40. https://doi.org/10.33837/msj.v1i7.339

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Technical Communications

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